| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1211 | Leptospira biflexa | AGAACGGTCGTGATTCGGT | ATCCCGTCAACGAGTGCA | 59.04 | 58.94 | 166 | 84 |
100.00%
|
100.00%
|
| Primer 1212 | Leptospira biflexa | TTCGTGCCGTTGCCAAAC | TCCCAATCGAGACGGGAAC | 59.59 | 59.10 | 210 | 84 |
100.00%
|
100.00%
|
| Primer 1213 | Leptospira biflexa | ACGGAAGTGGTTCACGGAC | TTGCCGTTTGGAGCCGAA | 59.93 | 60.20 | 185 | 84 |
100.00%
|
100.00%
|
| Primer 1214 | Leptospira biflexa | CGGAAGTGGTTCACGGACT | TTGCCGTTTGGAGCCGAA | 59.63 | 60.20 | 184 | 84 |
100.00%
|
100.00%
|
| Primer 1215 | Leptospira biflexa | ACGGAAGTGGTTCACGGA | TTGCCGTTTGGAGCCGAA | 58.46 | 60.20 | 185 | 84 |
100.00%
|
100.00%
|
| Primer 1216 | Leptospira biflexa | CTGGTGGGGAAGGCGAAAT | CGAAAGTTCGTCCCCACCA | 60.00 | 59.93 | 206 | 84 |
100.00%
|
100.00%
|
| Primer 1217 | Leptospira biflexa | CCTTTTGCCTCCACCTGGT | CGAAAGTTCGTCCCCACCA | 59.85 | 59.93 | 220 | 84 |
100.00%
|
100.00%
|
| Primer 1218 | Leptospira biflexa | TGGTGGGGAAGGCGAAAT | CGAAAGTTCGTCCCCACCA | 58.82 | 59.93 | 205 | 84 |
100.00%
|
100.00%
|
| Primer 1219 | Leptospira biflexa | ACGCAAACTGACTTCCGGT | TCATGCCCATGGTGTCCA | 59.85 | 58.49 | 219 | 84 |
100.00%
|
100.00%
|
| Primer 1220 | Leptospira biflexa | ACGCAAACTGACTTCCGGT | TGCCCATGGTGTCCAAACT | 59.85 | 59.46 | 216 | 84 |
100.00%
|
100.00%
|